Phenolic Response to Walnut Anthracnose (Ophiognomonia leptostyla) Infection in Different Parts of Juglans regia Husks, Using HPLC-MS/MS

This study compares the individual phenolic response of husk tissues Juglans regia L., infected to different degrees severity with walnut anthracnose, which is one most serious and widespread diseases worldwide. A comparison among three differently susceptible cultivars, ‘Franquette’, ‘Milotai 10’ (‘M10’), intenziv’ (‘M10-37’), made. In our methodology, high performance liquid chromatography coupled mass spectrometry used identify quantify compounds. Our results show that flavanols, flavonols, naphthoquinones account for more than 95% compounds identified in husk. The higher total analyzed content affected by anthracnose confirmed phenolics play a major role plant’s against pathogens. difference between cultivars observed, since French cultivar ‘Franquette’ responds infection Hungarian ‘M10’ ‘M10-37’. Naphthoquinones flavanols have very similar infection. resistance may be due reaction time plant speed it recognizes pathogen quickly containing while has not yet spread. Flavonols important disease control, they respond rapidly naphthoquinones. They also an inhibitory early stages viral bacterial infections.